Booking NGS prometion24 â ī¸ Access Requirements Only CISUP members who are trained and recognized as experienced users can operate the PromethION 24 autonomously; there is no assigned technician. đ Booking Process Requests must be made at least 3 days in advance with exact dates and times, and they require confirmation from instrument managers to be valid. ⥠Capacity and Slots The instrument can run up to 3 concurrent experiments, with a limit of 8 flow cells per experiment. Using more than 8 flow cells requires booking two slots. Select your booking dates below: Calendar is loading...19- Available19- Booked19- Pending19- Partially available * = required fields 1. Booking Info Start time*: 09:0000:0001:0002:0003:0004:0005:0006:0007:0008:0010:0011:0012:0013:0014:0015:0016:0017:0018:0019:0020:0021:0022:0023:0000:00 End time*: 10:0000:0001:0002:0003:0004:0005:0006:0007:0008:0009:0011:0012:0013:0014:0015:0016:0017:0018:0019:0020:0021:0022:0023:0000:00 Affiliation*: UniPisaOther UniversitiesResearch InstitutesThird parties/Private companies Project/Quotation â: 2. Context Department: BiologiaChimica e Chimica IndustrialeCivilta' e Forme del SapereEconomia e ManagementFarmaciaFilologia, Letteratura e LinguisticaFisicaGiurisprudenzaInformaticaIngegneria Civile e IndustrialeIngegneria dell'Energia, dei Sistemi, del Territorio e delle CostruzioniIngegneria dell'InformazioneMatematicaMedicina Clinica e SperimentalePatologia Chirurgica, Medica, Molecolare e dell'Area CriticaRicerca Traslazionale e delle Nuove Tecnologie in Medicina e ChirurgiaScienze Agrarie, Alimentari e Agro-ambientaliScienze PoliticheScienze VeterinarieScienze della Terra Type of Activity*: Research â UniPisa staff and CISUP fellowsEducation â UniPisa staff classesResearch â External users from academiaThird partiesLab management - staff 3. Contact Information đ¤ Applicant (Richiedente) Name*: Email*: Phone: đŗ Fund Manager (Responsabile) Name*: Email*: Phone: Continue to step 2 â Sample Specifications Nucleic acid type * DNARNABoth (DNA + RNA) Number of samples * (Number of flow cells requested) Number of experiments / slots requested * âšī¸ Max 3 concurrent experiments; each experiment = 8 flow cells. More than 8 flow cells occupies two slots. 1 experiment (8 flow cells)2 experiments (16 flow cells)3 experiments (24 flow cells) Basecalling model required * âšī¸ Determines GPU load. More accurate models continue in "catch-up mode" after the run completes. FastHigh accuracy (HAC)Super accurate (SUP) Base modification / methylation calling (optional) (5mC, 5hmC, 6mA for DNA; m6A for RNA can be called during basecalling) YesNo Variant calling / downstream analysis (optional) (Indicate whether analysis will be performed downstream) SNVs / indelsStructural variantsMethylationRaw dataOther Note for applicants: The P24 carries four NVIDIA A-Series GPUs and can basecall in real time, but HAC keeps up in real time for up to 18 flow cells on the PromethION A-Series; with SUP, or with a higher number of flow cells, basecalling continues in catch-up mode after the run and occupies the instrument for longer. When selecting SUP, please include the expected catch-up time in your booked slot, in addition to the time required to download and remove the data from the instrument. I Accept term and conditions đ CISUP ByLaws â Back to step 1 Send Application
