Fluorescence Super-resolution Microscope – Nikon STORM 5.0

The super-resolution system Nikon STORM 5.0 provides a reliable single-molecule localization microscopy method to achieve super-resolution images (lateral resolution ~20nm and axial resolution ~50nm). The system employs total internal reflection fluorescence (TIRF), highly inclined and wide-field illumination for single molecule detection. Software allows single-molecule localization, image reconstruction and post-processing (STORM rendering and trajectories analysis).

Applications

> Intracellular proteins distribution (nuclear factors, cytoskeletal-associated proteins, molecular motors, focal adhesion complexes);
> Membrane proteins nano-organization and clustering;
> Intracellular transport studies and molecular motors imaging.

Technical specifications

> Microscope: ECLIPSE Ti2-E with Perfect Focus system;
> Max resolution (detector): 2304×2304 pixels (16bit), 6.5 µm x 6.5 µm pixels;
> Stage: High resolution motorized stage (TI2-S-SE-E) with linear encoder ;
> Sample holder: Universal adapter for Petri (35-60mm), slides and Labtek;
> Time lapse: ND acquisition ECLIPSE Ti2-E.

Objectives, filters, detector and lasers

> CFI Plan Apochromat Lambda 10X, CFI Plan Apochromat;
> 40X, Obiettivo CFI SR HP 100x apocromatico TIRF NA=1,49 WD=0,12 mm;
> CMOS back illuminated Orca Fusion BT (quantum efficiency >95%, 89 frames/sec);
> TIRF multiband dicroic filter 405/488/561/640;
> 405 nm, 488 nm, 561 nm, 647 nm.