Light-sheet Fluorescence Microscope – Aurora, MSquaredLife

The system Aurora (MSquaredLife) provides a reliable microscopy method, based on light sheet fluorescence illumination, able to achieve 3D volumetric imaging. Light-sheet illumination combines optical sectioning with fast imaging in tissues, living organisms and fixed samples. The planar illumination is obtained by Airy beam structured illumination, improving the field of view reachable and the imaging depth capabilities. Objective lenses with long working distance (10mm) provide a lateral resolution of ~500nm (@525nm).

Applications

> 3D multicolor imaging of live cells, organoids and tissues;
> Imaging of microanatomy of human brain organoids derived from healthy and diseased patients;
> Observation of the volumetric arrangement of neuronal populations in murine and human tissues;
> Studying the cellular arrangement in human-relevant models of liver and barriers (alveolar, gut, ecc).

Technical specifications

> Microscope: automated filter-wheels, motorized stage;
> Max resolution (detector): 2304×2304 pixels (16bit), 6.5 µm x 6.5 µm pixels;
> Temperature control: 0°C-37°C;
> Sample holder: custom, compatible with different medium (acqueous, TDE);
> Workstation: Dual Intel Xeon Silver 4215 2.5GHz; NVIDIA Quadro P400; 192GB 2933MHz ECC memory; 40 TB RAID0 storage. Data storage (64 TB RAID0);
> Analysis: deconvolution, automatic calibration.

Objectives, filters, detector and lasers

> Detection lens with long WD (NA=0.64-0.75) – magnification 21.9x-25.6x);
> CMOS back illuminated Orca Fusion BT (quantum efficiency >95%, 89 frames/sec);
> CMOS back illuminated Orca Fusion BT (quantum efficiency >95%, 89 frames/sec);
> TIRF multiband dicroic filter 405/488/561/640;
> Diode laser: CW (60mW@405 nm), CW (100 mW @488 nm), CW (100 mW @561 nm), CW (100 mW @647 nm).