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Operetta High Content Imaging

Overview of A375 cells treated with different concentrations of Chloroquine and stained with anti-LC3 antibodyOverview of A375 cells treated with different concentrations of Chloroquine and stained with anti-LC3 antibody.
A375 cells treated with 10 µM ChloroquineA375 cells treated with 10 µM Chloroquine
A375 cells treated with 3 µM ChloroquineA375 cells treated with 3 µM Chloroquine
A375 cells treated with 0,3 µM ChloroquineA375 cells treated with 0,3 µM Chloroquine

Location: Retrovirus Center, Via del Brennero 2

Lab head: Dr. Michele Lai

Contact: michele.lai@unipi.it

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Annual report

The promise of high-content screening is the acceleration of discovery by extracting as much relevant information as possible from cells. Nevertheless, a large percentage of high-content screens analyse only a small number of image-based properties. As a result, valuable information from precious cells and disease models is not utilized. Operetta CLS high-content analysis system provides you the solution to monitor and evaluate your samples deeply. The instrument supports research from everyday assays to more demanding applications. It delivers a balance of flexible excitation, optics, and advanced software features to enable you to gain deeper biological insight from all your critical applications.
Fixed-cell assaysUp to eight high-power excitation sources and user-accessible emission filters enable maximum flexibility of fluorescent stains and labels, plus the system features widefield and spinning-disk fluorescent imaging.
Live-cell assaysThe spinning disk confocal optics and synchronized LED illumination provide stable excitation and minimize phototoxicity and bleaching for meaningful live-cell assays. You can also choose brightfield or digital-phase contrast imaging modes.
Complex cellular modelsThe large-format sCMOS camera with water-immersion objectives provides sensitivity and high resolution, while advanced software helps you address the imaging and analysis challenges presented by complex cellular models.
Advanced assaysFRET is a powerful tool for investigating conformational changes and protein-protein interactions. The Operetta CLS system’s sensitive imaging and dedicated analysis tools for ratiometric imaging, facilitate robust results.
Phenotypic fingerprintingThe Operetta CLS combines high resolution imaging with advanced software tools to help create robust phenotypic fingerprints of the subtle differences at the core of successful phenotypic assays.
Time Movie Operetta NPs Operetta (high content imaging) at Bright Night

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